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Background. Peptides are important components of foods mainly due to their biological activity. The basic method of their identification is reversed phase high-performance liquid chromatography coupled with electrospray-ionization mass spectrometry (RP-HPLC- -ESI-MS). Retention time (tR) prediction in silico is very helpful in analysis of multicomponent peptide mixtures. One of problems associated...
Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenotypic...
Biogenic amines can be naturally present in many foods and they can also be produced in high amounts by the activity of amino acid decarboxylases of microorganisms. If ingested in significant amounts they may produce direct or indirect effects on a consumer’s health. In food microbiology, their large presence has been associated to spoilage and fermentation processes. The aim of the present study...
Milk samples were taken from cows with acute, subacute, chronic, and subclinical mastitis and from healthy cows. The mean activity of lactoferrin (LF) in milk from mastitic cows ranged from 8.9 ±3.0 to 12.1 ±6.9 mU/g protein and was significantly lower than that in milk from healthy cows (29.5 ±15.0 mU/g protein). In group of mastitic cows the highest LF activity was found in cows with chronic mastitis,...
The paper presents the analytical procedure of organic acids quantification in compound feed by ion chromatography method. Organic acids: acetic, propionic, formic, malic, fumaric, and citric were extracted from a feed sample with water for 30 min. The measurement was done by conductometric detection. The limit of quantification of the assayed acids in the feeds was as follows: acetic 0.50 g/kg, propionic...
The aim of the research was the determination of resistance to fluoroquinolones (ciprofloxacin and enrofloxacin) of 387 Campylobacter sp. strains isolated from poultry between 1994-1996 (broilers, laying hens) and between 2005-2008 (broilers, laying hens, and turkeys). The resistance was determined by an agar dilution method (MIC), with accordance to Clinical and Laboratory Standards Institute recommendation...
A liquid chromatographic method coupled with UV-Vis and fluorescence detectors for the determination of azaperone, carazolol, and chlorpromazine residues in urine has been described. Analytes were isolated from biological samples with acetonitrvle. The obtained extracts were applied to solid phase extraction Strata X-C 33 µn Polimeric Strong Cation columns. The analytes were eluted with alkalised...
Metallothionein (MT) is a low molecular weight cysteine-rich protein with a number of roles in the pro/antioxidant balance and homeostasis of essential metals, such as zinc and copper, and in the detoxification of heavy metals, such as cadmium and mercury. Until now, detection of metallothionein in biological fluids remained difficult because of a lack of a broadly reactive commercial test. Meaningful...
One of the most frequently used methods for determining total mercury content in biological samples is cold vapour atomic absorption spectrometry (CV-AAS), which is extensively used in the biomonitoring of environmental pollution. Seabirds are often used as bioindicators of metal contamination because of their specific feeding habits, wide geographical ranges and long lifespan. This paper describes...
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