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The requirements for a polymerase chain reaction (PCR) microchip are reduction in reaction time and PCR reagent volume. In this paper, we describe the design and experimental studies of a droplet-based PCR by electrowetting on a dielectric (EWOD) chip by using the droplet basis to reduce PCR reagent volume. The droplet-based PCR system consists of a single-plate EWOD device and serpentine-shape microheaters...
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