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Membrane-bound macromolecules play an important role in tissue architecture and cell-cell communication, and is regulated by almost one-third of the genome. At the optical scale, one group of membrane proteins expresses themselves as linear structures along the cell surface boundaries, while others are sequestered. This paper targets the former group, whose intensity distributions are often heterogeneous...
Our objective is to develop a highly miniaturized, solid-phase platform for assaying the function and inhibition of integral membrane proteins (IMPs). To functionalize materials with active membrane proteins, the challenge is to build stabilized, supported biomembranes in which the substrate to biomembrane spacing can be controlled to accommodate larger membrane protein. The silica microsphere (5...
In this work we for the first time demonstrate real-time monitoring of the expression of membrane proteins in native, live cells, free of hydrodynamic stress at single cell resolution. This micro-optofluidic mechanism is uniquely enabled by the intricate interplay of gravity induced sedimentation with laminar flow, fast diffusion and short optical path length on our lab-in-a-trench platform.
Membrane protein interactions are involved in the regulation and execution of all biochemical pathways within the cell. It is crucial to identify the binding partners for understanding the function of an uncharacterized protein, and when these partners turn out to have known function, deductions about the potential role of the uncharacterized protein can often be made. However, the analysis of interactions...
Summary form only given: Although ionized gases have been known to have biological effects for more than 100 years, their impact on the practice in healthcare service became very significant only recently. Today, plasma-based surgical tools are used for tissue reduction and blood coagulation as surgical procedures. Most significant however is the speed at which low-temperature gas plasmas are finding...
Membrane proteins are an important class of proteins that serve as channels, receptors, and energy transducers in a cell membrane. Knowledge of a given type of cell membrane protein is crucial for determining its function. This paper introduces an automated, in-silico method for identifying different types of membrane proteins based on their amino acid composition. Our method applies a novel, composite...
Membrane proteins organize themselves in a linear fashion where adjacent cells are attached together along the basal-lateral region. Their intensity distributions are often heterogeneous and may lack specificity. Grouping of these linear structures can aid in segmentation and quantitative representation of protein localization. However, quantitative analysis of these signals is often hindered by noise,...
Giant unilamellar vesicles (GUVs) are biochemical compartments enclosed with phospholipid bilayers. GUVs are expected to be materials for construction and investigation of artificial cell models. There, fusion phenomena between GUVs and smaller vesicles (referred to as "asymmetric fusion" here) seem to have some potential for utilization of the transporters bringing reagents and membrane...
In the early seventies, it was clear that primary amino acid sequence and its local solution environment hold most of the information necessary for protein folding. Since then, scientists have been trying to solve the bioinformatics problem by constructing the tertiary three-dimensional structure of protein from the primary amino acid sequences by using computational biology. Success of several genome...
Molecular biology works at the nanoscale with most cellular processes depending on complexes of proteins, DNA and membranes that have dimensions in the range 1-100 nm. In order to interface with biology any physical device should present biological components which are ordered on this scale. To achieve this control we must resort to bottom-up methods of manufacturing and this requires that the biological...
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