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Due to the important physiological functions of GABA, various methods used to produce it. Biotransformation by microorganism cells is a more promising method than others. Among the GABA-producing lactic acid bacteria, one strain with high producing capacity was isolated from Chinese naturally pickled vegetable. The strain is identified as Lactobacillus brevis, and named as L.brevis TCCC13007 based...
In order to understand the phylogenetic relationships among Vibrio species more rationally , the hemolysin gene partial sequences of 4 Vibrio species (Vibrio alginolyticus, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio minius) were amplifyed and sequenced. We constructed two separate phylogenetic trees by using 16S rRNA and partial hemolysin gene sequences, respectively.The average similarities...
Xylanase (E.C 3.2.1.8) is a widespread group of enzyme which can catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in xylan. Xylanase has a wide application in industrial processes. In this study, the total RNA was isolated, the mature xylanase gene was obtained from Aspergillus niger C3486 by RT-PCR. To achieve secretive expression, pET32a (+) was used as expression vector, and the cloned...
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