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The cellular biocompatibility of two types of nanophase hydroxyapatites (HA and CDHA) synthesized by a wet chemical method was assessed using primary cultured osteoblasts. Cytotoxicity of both materials was investigated with L929 cell line. The MTT method was used to evaluate the proliferation of osteoblasts on the third day. SEM was used to observe the morphology of the osteoblasts on the third day...
In this paper, hydroxyapatite (HA) sol composed of nanosized HA particles was incorporated into collagen gel to regulate cell-mediated collagen contraction. Human bone marrow derived stromal cells (hMSCs) were cultured for 37 days in gels with HA to collagen ratios of 0:1, 0.5:1, 1:1, 1.5:1, and 2:1, respectively. The contraction of gels was evaluated by measuring diameter change with incubation time,...
A hybrid, nanosized, localized and release-controlled bone morphogenetic protein delivery system consisting of a liposomal core incorporated into a shell of alternating layer-by-layer self-assembled natural polyelectrolytes has been formulated. Hydrophilic, monodisperse, spherical and stable cationic nanoparticles with an extended shelf-life allowing immediate protein loading prior to clinical administration...
Before using semiconductor quantum dots (QDs) in biomedical applications in vivo, it's important to know about their interaction with biological surroundings, stability in living organisms, intracellular distribution and cytotoxicity. We have investigated CdTe-MPA and (CdSe)ZnS-MUA QDs in the osteosarcoma U2-OS cell culture. The changes in photoluminescence spectra of QDs, the localization of QDs...
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