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Noncanonical G‐C+ and A‐T Hoogsteen base pairs can form in duplex DNA and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in DNA duplexes remains challenging due to difficulties in resolving syn versus antipurine bases with X‐ray crystallography; and size limitations and line broadening can make them difficult to characterize by NMR spectroscopy. Here, we...
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