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Objective-- To explore the effects of siRNA targeting to Virion host shutoff protein (VHS) on HSV-1 replication in vero cell. Methods--Three kinds of siRNAs were Tran scripted in vitro using T7RNA polymerase according to the principle of siRNA design. The Vero cells were transfected with siRNA using lipofectamine 2000 followed by HSV-1 infection. The activity of vero cells were detected by MTT method...
RNA interference (RNAi) is a widely used method for gene function analyses in a variety of organisms. Establishment of a large-scale, high-throughput platform for analysing gene function may require development of an RNAi microarray system using reverse transfection. Here, we show that shRNA is more effective in microarray gene silencing compared to siRNA. Endogenous mammalian genes are suppressed...
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