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The amylomaltase gene of the Streptomyces ST66 was cloned. The open reading frame of this gene consisted of 2,136 nucleotides and encoded a polypeptide that was711 amino acids. The amylomaltase gene was overexpressed in E. coli. The detailed action profile of crude enzyme from E.coli Top 10F'/pTrc-CKS- MalQ on amylose was analyzed and tested whether this crude enzyme could catalyze cyclization of...
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