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Copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC) click reaction in the major groove of DNA containing 5‐ethynyluracil (UE) with azides was used for turning off sequence‐specific protein–DNA interactions. The concept was first demonstrated on switching off cleavage of short modified DNA by restriction endonuclease BamHI‐HF. Finally, DNA template containing UE was used for in vitro transcription...
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