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Gliadins, seed storage proteins, are popular markers effectively employed for the analysis of common wheat. Gliadin electrophoretic patterns are genotype-specific, reproducible, not dependent on growing conditions and are suitable for germplasm identification complementary to molecular markers. Gliadins have been identified and used in wheat from various countries, but prior to this study little was...
Flours of four common wheat and one spelt genotypes were treated with thioredoxin-h (TR-h) in the presence of NADPH and NADP-thiore- doxin reductase to reduce intramolecular disulphide bonds in monomeric sulphur-rich prolamins. Effects on the breadmaking properties (determined by micro-baking) and gliadin's immunoreactivity (measured by Sandwich ELISA) were observed. Loaf volumes and estimated crumb...
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