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The oligomers of Aβ1–40 peptide in PBS buffer solution were analyzed by SEC and native PAGE, and the trimer of Aβ1–40 was also isolated by SEC. In addition, the effects of the soluble Aβ1–40 trimer on intracellular free calcium (Ca2+) balance of hippocampal neurons of postnatal rats were investigated by fluorescence microscopy. The experimental results indicated that Aβ1–40 peptide existed in the...