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article

Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

Tania García-Mendiola, Cristina Garcia Elosegui, Iria Bravo, Félix Pariente, more

Microchimica Acta > 2019 > 186 > 5 > 1-8

The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the...

article

Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future

Ameh S. James, Shawn Todd, Nina M. Pollak, Glenn A. Marsh, more

Virology Journal > 2018 > 15 > 1 > 1-9

Background The 2014/2015 Ebolavirus outbreak resulted in more than 28,000 cases and 11,323 reported deaths, as of March 2016. Domestic transmission of the Guinea strain associated with the outbreak occurred mainly in six African countries, and international transmission was reported in four countries. Outbreak management was limited by the inability to rapidly diagnose infected cases. A further fifteen...

article

Performance characteristics of an automated latex immunoturbidimetric assay [HemosIL® HIT-Ab(PF4-H)] for the diagnosis of immune heparin-induced thrombocytopenia

Theodore E. Warkentin, Jo-Ann I. Sheppard, Lori-Ann Linkins, Donald M. Arnold, more

Thrombosis Research > 2017 > 153 > C > 108-117

Heparin-induced thrombocytopenia (HIT) is a prothrombotic drug reaction caused by platelet-activating anti-PF4/heparin antibodies. Given time-sensitive treatment considerations, a rapid and accurate laboratory test for HIT antibodies is needed.To determine operating characteristics for the HemosIL^® HIT-Ab_(PF4/H), a rapid, on-demand, fully-automated, latex immunoturbidimetric assay (LIA), for diagnosis...

article

Pediatric tuberculosis-human immunodeficiency virus co-infection in the United Kingdom highlights the need for better therapy monitoring tools: a case report

Dimitrios Evangelopoulos, Elizabeth Whittaker, Isobella Honeyborne, Timothy D. McHugh, more

Journal of Medical Case Reports > 2017 > 11 > 1 > 1-4

Background Tuberculosis is an infection that requires at least 6 months of chemotherapy in order to clear the bacteria from the patient’s lungs. Usually, therapeutic monitoring is dependent on smear microscopy where a decline in acid-fast bacilli is observed. However, this might not be indicative of the actual decline of bacterial load and thus other tools such as culture and molecular assays are...

chapter

A compound rapid assay method for crude oil properties based on similarity discrimination and partial least squares

Xisong Chen, Mi Du, Jun Yang

2016 35th Chinese Control Conference (CCC) > 2029 - 2033

2016 35th Chinese Control Conference (CCC)

The measurement of crude oil properties is of paramount importance to oil processing in refinery plants. Although some methods for the rapid assay of crude oil properties have been proposed, the measurement accuracy is still not high enough in some cases. A compound rapid assay method for crude oil properties based on similarity discrimination and partial least squares (PLS) is put forward in this...

article

Alternative methods to determine infectivity of Tulane virus: A surrogate for human nororvirus

Shuxia Xu, Dapeng Wang, David Yang, Huashan Liu, more

Food Microbiology > 2015 > 48 > Complete > 22-27

Culturable animal caliciviruses are widely-used as surrogates for human norovirus (HuNoV). The infectivity of a culturable virus was traditionally determined by plaque assay and/or 50% tissue culture infectious dose (TCID ₅₀ ) assay, both of which are time-consuming and labor-intensive. Molecular approaches, such as quantitative real time RT-PCR (qRT-PCR) and RT-PCR, could be used for detection...

article

Rapid high-throughput assay to assess scavenging capacity index using DPPH

Fatima Abderrahim, Silvia M. Arribas, M. Carmen Gonzalez, Luis Condezo-Hoyos

Food Chemistry > 2013 > 141 > 2 > 788-794

A new microplate-adapted DPPH rapid assay was developed to assess the antioxidant capacity of pure compounds and foods. The assay was carried out in buffered medium (methanol: 10mmol/l Tris buffer pH 7.5, 1:1 v/v) and reaction was completed at 10min. The scavenging capacity index (SCI), a theoretical antioxidant parameter directly related to the antioxidant capacity of samples, was calculated. SCI...

article

A rapid assay for on-site monitoring of infliximab trough levels: a feasibility study

Paul L. A. M. Corstjens, Herma H. Fidder, Karien C. Wiesmeijer, Claudia J. Dood, more

Analytical and Bioanalytical Chemistry > 2013 > 405 > 23 > 7367-7375

Monitoring levels of biologicals against tumor necrosis factor (TNF) has been suggested to improve therapeutic outcomes in inflammatory bowel diseases (IBDs). This pilot study describes a rapid lateral flow (LF)-based assay for on-site monitoring of serum trough levels of humanized monoclonal antibody infliximab (IFX). The applied chromatographic method utilizes sequential flows of diluted serum,...

article

New Sandwich‐Type Enzyme‐Linked Immunosorbent Assay for Human MxA Protein in a Whole Blood Using Monoclonal Antibodies Against GTP‐Binding Domain for Recognition of Viral Infection

Mizuho Kawamura, Akira Kusano, Akiko Furuya, Nobuo Hanai, more

Journal of Clinical Laboratory Analysis > 26 > 3 > 174 - 183

ObjectivesTo develop a clinically significant and practical enzyme‐linked immunosorbent assay (ELISA) for the detection of MxA protein in human whole blood, a biological marker of viral infection. Design and MethodsA sandwich ELISA suitable for the measurement of human MxA protein in whole blood was developed using mouse monoclonal antibodies (mAbs) raised against the GTP‐binding domain of human...

article

Dipstick immunoassay for rapid diagnosis of paratuberculosis in small ruminants

K. Karthik, Praveen Singh, P. Das

Small Ruminant Research > 2011 > 99 > 2-3 > 214-221

Highly sensitive and specific gold nanoparticle based dipstick immunoassay using protoplasmic antigen of Mycobacterium avium subspecies paratuberculosis (MAP) for diagnosis of paratuberculosis was introduced. In this method the colloidal gold nanoparticles (GNPs) were coated with MAP protoplasmic antigen using alkanethiols derivatives and anti-MAP rabbit antibodies. These antigen coated GNPs acts...

article

Comparison of the NIDS® rapid assay with ELISA methods in immunogenicity testing of two biotherapeutics

Jing Pan, Thomas Small, Dujie Qin, Shawn Li, more

Journal of Pharmacological and Toxicological Methods > 2011 > 63 > 2 > 150-159

Rapid lateral flow immunogenicity assays for the detection of anti-drug antibodies (ADAs) to two biotherapeutic antibodies, an anti-HER2 antibody and an anti-TNF-α antibody, were developed using ANP Technologies, Inc.'s proprietary Nano-Intelligent Detection System (NIDS®) and compared to their ELISA counterparts.Biotin and hapten-labeled drugs are incubated with the patient serum sample to allow...

article

Detection of somatic coliphages through a bioluminescence assay measuring phage mediated release of adenylate kinase and adenosine 5′-triphosphate

Carolina Guzmán Luna, Ana Costán-Longares, Francisco Lucena, Joan Jofre

Journal of Virological Methods > 2009 > 161 > 1 > 107-113

The feasibility of detecting somatic coliphages by phage infection of Escherichia coli WG5 and measurement of phage propagation by the lysis mediated release of the bacterial host adenylate kinase (AK) and adenosine 5′-triphosphate (ATP) detected by a bioluminescent signal was evaluated. After 2h of incubation, all cultures infected with reference bacteriophage ϕX174 showed a significant increase...

article

Rapid DNA multi-analyte immunoassay on a magneto-resistance biosensor

M. Koets, T. van der Wijk, J.T.W.M. van Eemeren, A. van Amerongen, more

Biosensors and Bioelectronics > 2009 > 24 > 7 > 1893-1898

We present the rapid and sensitive detection of amplified DNA on a giant magneto-resistance sensor using superparamagnetic particles as a detection label. The one-step assay is performed on an integrated and miniaturized detection platform suitable for application into point-of-care devices. A double-tagged PCR amplification product of the LamB gene of the Escherichia coli bacterium was used to investigate...

article

Analysis of total aerobic viable counts in samples of raw meat using fluorescence-based probe and oxygen consumption assay

Fiach O’Mahony, Rebecca A. Green, Chris Baylis, Richard Fernandes, more

Food Control > 2009 > 20 > 2 > 129-135

A simple test for determining total aerobic viable counts in raw meat is presented. Homogenates of meat samples are prepared in full PBW medium, dispensed in the wells of 96-well plate together with the oxygen-sensing probe, Redlight, covered with oil and monitored on a fluorescent reader at 30°C. The probe produces characteristic sigmoidal profiles of fluorescence reflecting depletion of sample dissolved...

article

Performance of a new immunochromatographic assay for detection of adenoviruses in children

Fatma Levent, Jewel M. Greer, Malorie Snider, Gail J. Demmler-Harrison

Journal of Clinical Virology > 2009 > 44 > 2 > 173-175

Adenoviruses are a prominent cause of respiratory, ocular, gastrointestinal, and disseminated diseases in healthy and immunocompromised children. An accurate rapid diagnostic assay may impact clinical decision-making.Evaluate the performance of a new rapid assay for detection of adenoviruses directly in pediatric clinical specimens.The rapid assay was performed on adenovirus culture-positive original...

article

Comparison of BD Directigen™ EZ RSV and Binax NOW® RSV tests for rapid detection of respiratory syncytial virus from nasopharyngeal aspirates in a pediatric population

Rangaraj Selvarangan, David Abel, Marilyn Hamilton

Diagnostic Microbiology & Infectious Disease > 2008 > 62 > 2 > 157-161

The BD Directigen™ EZ RSV (BD) assay and the Binax NOW ^® RSV (BN) assays are lateral flow immunochromatographic assays used in the rapid diagnosis of respiratory syncytial virus (RSV) infection. A prospective study was undertaken to compare the performance characteristics of the BD and BN assays using 99 fresh nasopharyngeal aspirate specimens from children. All specimens were cultured by...

article

Rapid assays for the diagnosis of influenza A and B viruses in patients evaluated at a large tertiary care children's hospital during two consecutive winter seasons

Andrea T. Cruz, Andreea C. Cazacu, Jewel M. Greer, Gail J. Demmler

Journal of Clinical Virology > 2008 > 41 > 2 > 143-147

The rapid and accurate diagnosis of influenza facilitates antiviral therapy, judicious antibiotic usage, and cohorting patients to decrease nosocomial infection.To determine the utility of rapid influenza tests in a children's hospital.Two in vitro rapid immunochromatographic assays that detect and distinguish influenza A and B viruses were compared to the reference standard of viral culture.In 9186...

article

Specific immunoassays for endocrine disruptor monitoring using recombinant antigens cloned by degenerated primer PCR

Natalia Ferraz, Daniel Carnevia, Gonzalo Nande, Martin Rossotti, more

Analytical and Bioanalytical Chemistry > 2007 > 389 > 7-8 > 2195-2202

Vitellogenin (VTG) and choriogenin (CHO) are valuable biomarkers of endocrine-disrupting compound (EDC) exposure in fish. Existing immunoassays are limited to a few species, which restricts their use for the analysis of local wildlife sentinels. Using C. facetum as a relevant South American model fish, this work presents a new strategy for the preparation of antibodies to VTG and CHO, with zero cross-reactivity...

article

Comparison of immunoassay and gas chromatography/mass spectrometry methods for measuring 3,5,6-trichloro-2-pyridinol in multiple sample media

Jane C. Chuang, Jeanette M. Van Emon, Allen W. Reed, Nadia Junod

Analytica Chimica Acta > 2004 > 517 > 1-2 > 177-185

Two enzyme-linked immunosorbent assay (ELISA) methods were evaluated for the determination of 3,5,6-trichloro-2-pyridinol (3,5,6-TCP) in multiple sample media (dust, soil, food, and urine). The dust and soil samples were analyzed by a commercial RaPID immunoassay testing kit. The food and urine samples were analyzed by a laboratory-based 96-microwell plate immunoassay format. Methanol was used as...

article

Rapid quantitative assay for acaricidal effects on Sarcoptes scabiei var. suis and Otodectes cynotis

L. Brimer, H. Bak, S.Aa. Henriksen

Experimental and Applied Acarology > 2004 > 33 > 1-2 > 81-91

Brimer et al. (Vet. Parasitol. 51: 123–135, 1993 and 59: 249–255, 1995) developed a migration assay for acaricidal effect of acetylcholinesterase inhibitors and macrocyclic lactones utilising Sarcoptes scabiei var. suis mites. In contrast to many others, this assay is fully quantitative but quite time-consuming. The aim of the present investigation was to modify this assay to become faster and simpler...

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Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future

Performance characteristics of an automated latex immunoturbidimetric assay [HemosIL® HIT-Ab(PF4-H)] for the diagnosis of immune heparin-induced thrombocytopenia

Pediatric tuberculosis-human immunodeficiency virus co-infection in the United Kingdom highlights the need for better therapy monitoring tools: a case report

A compound rapid assay method for crude oil properties based on similarity discrimination and partial least squares

Alternative methods to determine infectivity of Tulane virus: A surrogate for human nororvirus

Rapid high-throughput assay to assess scavenging capacity index using DPPH

A rapid assay for on-site monitoring of infliximab trough levels: a feasibility study

New Sandwich‐Type Enzyme‐Linked Immunosorbent Assay for Human MxA Protein in a Whole Blood Using Monoclonal Antibodies Against GTP‐Binding Domain for Recognition of Viral Infection

Dipstick immunoassay for rapid diagnosis of paratuberculosis in small ruminants

Comparison of the NIDS® rapid assay with ELISA methods in immunogenicity testing of two biotherapeutics

Detection of somatic coliphages through a bioluminescence assay measuring phage mediated release of adenylate kinase and adenosine 5′-triphosphate

Rapid DNA multi-analyte immunoassay on a magneto-resistance biosensor

Analysis of total aerobic viable counts in samples of raw meat using fluorescence-based probe and oxygen consumption assay

Performance of a new immunochromatographic assay for detection of adenoviruses in children

Comparison of BD Directigen™ EZ RSV and Binax NOW® RSV tests for rapid detection of respiratory syncytial virus from nasopharyngeal aspirates in a pediatric population

Rapid assays for the diagnosis of influenza A and B viruses in patients evaluated at a large tertiary care children's hospital during two consecutive winter seasons

Specific immunoassays for endocrine disruptor monitoring using recombinant antigens cloned by degenerated primer PCR

Comparison of immunoassay and gas chromatography/mass spectrometry methods for measuring 3,5,6-trichloro-2-pyridinol in multiple sample media

Rapid quantitative assay for acaricidal effects on Sarcoptes scabiei var. suis and Otodectes cynotis

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