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To better understand the role of host cell‐derived molecules on enterohemorrhagic Escherichia coli (EHEC) infection, we studied EHEC virulence gene expression when exposed to cell‐free spent (conditioned) medium (CM) from HCT‐8 intestinal epithelial cells. Exposure to HCT‐8 CM for 1 h and 3 h increased the expression of 32 of 41 EHEC locus of enterocyte effacement (LEE) virulence genes compared with...