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We demonstrate the use of shaped 10 fs pulses for multimodal microscopy. The combination of a broadband oscillator and a pulse shaper provides a flexible light source that can be optimized for various nonlinear effects produced in the sample either for signal intensity or selectivity. Switching between narrowband and broadband Multiplex CARS, elimination of unwanted two-photon fluorescence or direct...
Pathological understanding of arterial diseases is mainly attributable to histological observations based on conventional tissue staining protocols. The emerging development of nonlinear optical microscopy (NLOM), particularly in second-harmonic generation, two-photon excited fluorescence and coherent Raman scattering, provides a new venue to visualize pathological changes in the extracellular matrix...
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