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As a model thermophilic bacterium for the production of second‐generation biofuels, the metabolism of Clostridium thermocellum has been widely studied. However, most studies have characterized C. thermocellum metabolism for growth at relatively low substrate concentrations. This outlook is not industrially relevant, however, as commercial viability requires substrate loadings of at least 100 g/L...
Given its availability, low prices, and high degree of reduction, glycerol has become an ideal feedstock for producing reduced compounds via anaerobic fermentation. We recently identified environmental conditions enabling the fermentative metabolism of glycerol in E. coli, along with the pathways and mechanisms mediating this metabolic process. In this work, we used the knowledge base created in previous...
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