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Cytochrome P450s belong to a family of heme‐binding monooxygenases, which catalyze regio‐ and stereospecific functionalisation of C–H, C–C, and C–N bonds, including heteroatom oxidation, oxidative C–C bond cleavages, and nitrene transfer. P450s are considered useful biocatalysts for the production of pharmaceutical products, fine chemicals, and bioremediating agents. Despite having tremendous biotechnological...
In the current work, we describe the identification and characterization of the first plasmid-encoded P450 (CYP107DY1) from a Bacillus species. The recombinant CYP107DY1 exhibits characteristic P450 absolute and reduced CO-bound difference spectra. Reconstitution with different redox systems revealed the autologous one, consisting of BmCPR and Fdx2, as the most effective one. Screening of a library...
Proliferating cell nuclear antigen (PCNA) from Sulfolobus solfataricus is a heterotrimeric ring-shaped protein. Bacterial cytochrome P450 (P450) that is one of promising biocatalysts requires electron transfer via two kinds of electron transfer proteins. Here we report that the fusion proteins of three subunits of PCNA and a bacterial P450 and its electron transfer proteins formed a quite stable heterotrimeric...
Molecular evolution is a powerful tool for improving or changing activities of enzymes for their use in biotechnological processes. Cytochromes P450 are highly interesting enzymes for biotechnological purposes because they are able to hydroxylate a broad variety of substrates with high regio- and stereoselectivity. One promising steroid hydroxylating cytochrome P450 for biotechnological applications...
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