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Two-step, low-pressure liquid chromatography has been used to isolate crocins, the most abundant compounds in the hydroalcoholic extract of saffron. A mixture of styrenedivinylbenzene copolymer and modified starch polymers was used as stationary phase. A 600 mm 15 mm i.d. column was used in the first step; the best resolution was achieved by gradient elution with an aqueous methanol gradient with...