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In this paper, a novel pretreatment approach, named SO3 micro-thermal explosion, greatly improved the saccharification of wheat straw was conducted for the first time although this method has been effectively applied for the rice straw. After combing with dilute alkali, the pretreated wheat straw contained 59.9% cellulose, 19.3% hemicellulose and 8.7% lignin, which means 77.5% of lignin was removed...