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An enumeration method to be used as an official control method in the framework of Council Directive 70/524/EEC for probiotic bifidobacteria used as feed additives was validated. Seventeen laboratories in 11 European Countries carried out a collaborative study. A spread plate method following BS ISO 15214:1998 using four different agars, Man Rogosa Sharpe (MRS), acidified MRS, MRS with triphenyl tetrazolium...