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We characterized 19 polymorphic microsatellite loci in the Neotropical butterfly Dione moneta. Based on the genotypes of 20 individuals from one population, we detected one to 13 alleles per locus, observed heterozygosities ranging from 0.00 to 0.80, and expected heterozygosities ranging from 0.23 to 0.88. Levels of variation were high, although heterozygosity deficiencies were found at most loci...