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Oxidasen sind bedeutsam für die chemische und pharmazeutische Industrie, da sie hochselektive Oxidationen katalysieren. Allerdings müssen natürlich vorkommende Oxidasen für synthetische Anwendungen häufig mittels Gelenkter Evolution im Labor maßgeschneidert werden. Hierfür haben wir die breit anwendbare und robuste Durchmusterungs‐Plattform “FlOxi” entwickelt. FlOxi ist eine Durchflusszytometriemethode...
Oxidases are of interest to chemical and pharmaceutical industries because they catalyze highly selective oxidations. However, oxidases found in nature often need to be re‐engineered for synthetic applications. Herein, we developed a versatile and robust flow cytometry‐based screening platform “FlOxi” for directed oxidase evolution. FlOxi utilizes hydrogen peroxide produced by oxidases expressed in...
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