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The basicity of highly protonated cytochrome c (cyt c) and myoglobin (myo) ions were investigated using tandem mass spectrometry, ion–molecule reactions (IMRs), and theoretical calculations as a function of charge state. Surprisingly, highly charged protein ions (HCPI) can readily protonate non‐polar molecules and inert gases, including Ar, O2, and N2 in thermal IMRs. The most HCPIs that can be observed...
Hochgeladene Protein‐Ionen sind ausreichend acide, um Edelgase wie Argon (Ar) in Ion‐Molekül‐Reaktionen bei Raumtemperatur zu protonieren. Diese Ionen sind die am stärksten aciden Moleküle, die jemals isoliert und detektiert wurden. In ihrer Zuschrift auf S. 8642 ff. berichten W. A. Donald et al. über Protein‐Supersäuren, die durch Elektrospray‐Ionisierung stark aufgeladen werden, wobei Proteine von...
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