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A new method was developed to determine the mutagenic efficacy of a suspected mutagen by employing green fluorescent protein (GFP) as a direct biosensor for mutation detection. Alterations in target gene (AcGFP1) expression after mutagen [(±)-7p,8a-dihydroxy-9a,10a-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE)] treatment were measured to detect the mutagenic efficacy of the carcinogen. In contrast...
Simple reagentless immunosensor formats have been difficult to achieve, particularly for electrochemical devices, since antigen/hapten recognition by an antibody does not directly lead to a reaction cascade. A direct reading electrochemical immunosensor would have major advantages with respect to speed, de-skilled analysis and the development of multi-analyte sensors.Electrically conducting polymers,...
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