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The “click chemistry” approach utilizing 5‐ethynyl‐2′‐deoxyuridine (EdU) as a DNA precursor was recently introduced to assess DNA replication and adapted to flow‐ and imaging‐cytometry. In the present study, we observed that EdU, once incorporated into DNA, induces DNA damage signaling (DDS) such as phosphorylation of ATM on Ser1981, of histone H2AX on Ser139, of p53 on Ser15, and of Chk2 on Thr68...