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In article number 1900030, Jingxin Meng, Shutao Wang, and co‐workers prepare non‐adhesive templates with defined geometries by laser etching, which can be used to controllably organize 3D cellular aggregates in vitro. The self‐organization of cells from simple to complex aggregates reveals the synergistic effect of multiscale topography from confined templates at the macroscale and cell interactions...
Self‐organization is a fundamental and indispensable process in a living system. To understand cell behavior in vivo such as tumorigenesis, 3D cellular aggregates, instead of 2D cellular sheets, have been employed as a vivid in vitro model for self‐organization. However, most focus on the macroscale wetting and fusion of cellular aggregates. In this study, it is reported that self‐organization of...
This paper proposes a capture device to manipulate and transport a cellular aggregate in a micro-well. A cellular aggregate (a few hundreds μm in diameter) is currently manipulated by a pipette. The manual manipulation by a pipette has problems; low reliability, low throughput, and difficulty in confirmation of task completion. We took into account of compatibility with existing methods such as a...
We have developed a polydimethylsiloxane (PDMS) pattern with arrays of microwells for the formation of multicellular aggregates by C17.2 neural stem cells. Upon interfacing with the patterns, the neural stem cells would firstly attach to the microwell sidewalls, forming cellular strips on day 1 after plating. For channel connected microwells, cellular strips on the concave semi-cylindrical sidewall...
This paper proposes a catcher device to manipulate and transport a cellular aggregate in a micro well. A cellular aggregate (around 100 μm in diameter) is currently manipulated by a pipette. Manual manipulation by a pipette has problems; low reliability, low throughput, and difficulty in confirmation of task completion. μTAS technology, especially various particle manipulation techniques on a fluidic...
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