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By immunological screening of a cDNA library constructed from potato tuber poly(A) + RNA and Escherichia coli expression vector pUC8 by the vector-primer and linker procedure of Okayama and Berg [(1982) Mol. Cell Biol. 2, 161-170], nearly full-length cDNA clones for patatin, a major protein of potato tuber, were identified. The cDNA carrying part of the 5'-noncoding region of the patatin mRNA,...
Purified and intact mitochondria isolated from potato tuber show a specific concanavalin A (Con A) binding as deduced from fluorescence labelling studies in the presence of the specific sugar α-D-methylmannose. This specific binding also occurs in mitoplasts, indicating the presence of Con A-binding sites on the inner mitochondrial membrane. In contrast, the binding of wheat germ agglutinin appears...
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