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Maintenance of immunopanned cells in culture medium in the absence of serum or pre-conditioning by other neural cell types such as astrocytes can be problematic. Here we report the novel use of a chemically defined medium, which we refer to as NBN since it contains N-2 supplement, B-27 supplement, and N-acetyl-l-cysteine, for maintaining O4+/O1- immunopanned pro-oligodendroglia. Since we had previously...
Primary culture of postnatal brainstem neurons in defined medium has not been described in the literature. Successful primary culture of brainstem neurons is typically restricted to embryonic ages E14-E18. This study describes a method for culture of late fetal and early postnatal brainstem neurons using a serum-free culture medium. The culture system is based on Neurobasal medium supplemented with...
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