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The vacuolar Zn 2+ /H + antiporter of Arabidopsis thaliana, AtMTP1, has a cytosolic histidine-rich loop (His-loop). We characterized the structures and Zn 2+ -binding properties of the His-loop and other domains. Circular dichroism analyses revealed that the His-loop partly consists of a polyproline type II structure and that its conformational change is induced by Zn 2+...
Cation diffusion facilitators (CDFs) have been described as requiring the C-terminal cytoplasmic domain for their function. With the identification of smaller proteins lacking the cytoplasmic portion but displaying sequential characteristics of CDFs, this assumption should be reconsidered. Here we describe the results showing that the MmCDF3, a 23-kDa protein lacking a C-terminal domain, interacts...
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