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Twelve Clostridium perfringens strains were isolated in 12 calves that had died with diarrhoea on two farms. On the basis of seroneutralization of antitoxins by diagnostic sera, 10 strains were classified into serotype A and 2 into serotype C. Most of the strains (7) were isolated from the small intestines of calves that died 7-18 h from the onset of diarrhoea. Characterization of the toxins revealed...
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