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The transesterification of high oleic sunflower oil with butanol by the immobilized Lipozyme® in n-hexane was carried out in a continuous packed bed reactor, oleic acid, butyl ester, and glycerol being formed as the main products. It was found that glycerol, insoluble in n-hexane, remained in the reactor adsorbed onto the enzymatic support, leading to a drastic decrease in enzymatic activity. The...
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