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Introduction: iloprost is a recommended treatment for several vascular diseases such as severe Raynaud's syndrome and digital ulcers in Systemic Sclerosis (SS). In vivo reflectance confocal microscopy (RCM) is a non-invasive imaging tool that can evaluate cutaneous microcirculation. The goal of this study was to observe, in real time, the effect of iloprost on cutaneous microcirculation by using RCM...
We address deconvolution and segmentation of blurry images. We propose to use Fuzzy C-Means (FCM) for regularizing Maximum Likelihood Expectation Maximization deconvolution approach. Regularization is performed by focusing the intensity of voxels around cluster centroids during deconvolution process. It is used to deconvolve extremely blurry images. It allows us retrieving sharp edges without impacting...
The analysis of F-actin organization in cells is a key topic in many fields of biomedical research. While standard protocols for imaging immunostained actin are well established, assessment of the resulting microscopy images is most of the time still performed manually and with a high degree of subjectivity. In this paper, we present a new approach for the analysis of actin structures in microscopy...
Determining the extent of liver fibrosis has clinically been difficult due to the lack of a simple, objective method that can accurately quantify the amount of collagen in the diseased tissue. Second harmonic generation (SHG) microscopy has been shown to produce bright and robust signals from non-centrosymmetric fibrillar collagen. We designed a SHG system that can objectively measure collagen presence...
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