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MRMPath, a system which processes protein sequences to create theoretical masses of peptide and their peptide fragments following digestion of the protein was developed. Experimentally, these fragments are created using enzymes prior to being processed in a mass spectrometer. In MRMPath, these fragments are created theoretically. MRMPath can process protein sequences from three different sources:...
To construct recombinant clone of glutathione S-transferase (GSTs) of Schistosoma japonicum (Sj) and express recombinant SjGSTs protein with his-tag that could be purified by single-step Ni2+-NTA affinity chromatography, one pair of primers was designed according to the sequence of GSTs gene. The DNA fragment of PGEX-KG GSTs gene was amplified by PCR with template PGEX-KG. Then it was cloned into...
Hydrogenase is the key terminal enzyme in electron delivery within hydrogen metabolism in bacteria, which has been attracted many attentions. In the present study, crude enzyme was firstly prepared by cell broken. Then a kind of hydrogenase from Ethanoligenens harbinense YUAN-3 was purified by chromatography methods on Sephadex G-100 and DEAE 52 columns. The enzyme was purified 169-fold with 7.8%...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
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