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Chitin deacetylase (CDA) is an enzyme that catalyzes the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in chitin. Chitin deacetylase converting chitin to chitosan in fungal cell walls. A total of 208 strains was isolated from soil samples in the riverbank of Xiangjiang River and an island of Zhanjiang. 12 CDA producing strains was founded by color reaction that including three fungus, two...
The DPV UL46 gene( GenBank accession No. EU195108) isolated by our laboratory was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmid pMD18-T-UL46 was identified by PCR, restriction enzymes (BamHI+XhoI), sequencing and nucleic acid dot-hybridization. Then the gene was analyzed by bioinformatics tools. The results showed that a 2220bp complete open reading frame of 739 amino acids...
Nitrite reductase (nirS) is a key enzyme of denitrification catalyzing the one electron reduction of nitrite (NO2-) to nitrogen monoxide (NO).In this study, nirS gene was cloned from Pseudomonas aeruginosa HGP9 strain. The phylogenetic tree was constructed and the secondary structure was predicted by bioinformatics. Results showed that nirS gene was 99.8% similar to the nitrite reductase from Pseudomonas aeruginosa...
Lipase activity is popular in staphylococci. We described here that a 781-bp consensus lipase gene sequence of Staphylococcus caprae TCCC 11546, which encoded a deduced polypeptide of 260 amino acid residues, was cloned using the degenerate primers. The amino acid sequence alignment and the phylogenetic tree revealed that this 260-amino acid partial lipase of S. caprae was highly homologous from 55...
Nucleotide sequences of the 16S rRNA gene of Thermobifida (formerly Thermomonospora) Q-0 was analyzed by MEGA v.3, and the phylogenetic tree showed the relationship between different strains in Thermobifida genus. A carboxymethyl cellulase I (CMCase I) was purified by fractional ammonium sulphate precipitation and ion exchange chromatography on DEAE-Sephadex. The enzyme showed the highest activity...
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