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The effect of chitosan (2 mg/ml) on the functionality of the plasma membrane of the Rhizopus stolonifer was studied. This study focuses on the changes in the integrity of the plasma membrane, external minimum medium pH, membrane potential, potassium efflux and determination of membrane phospholipids and proteins and of the H+‐ATPase enzymatic kinetic activity. The results demonstrated that the membrane...
Here we present a new technique to monitor dynamic conformational changes in voltage-sensitive membrane-bound proteins using radio frequency (RF) impedance measurements. Xenopus oocytes were transfected to express ShakerB-IR K+ ion channels, and step changes in membrane potential were applied using two-electrode voltage clamp (TEVC). Simultaneously, bipolar extracellular electrodes were used to measure...
To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+]i in the cells are observed using LCSM. The results of double staining with TMRE and...
Pf3 coat protein, a protein coded by bacteriophage Pf3, is a short membrane spanning protein consisting from 44 amino acid residues. Its mutant protein PO-3L, having three additional Leu residues in its transmembrane region, was demonstrated to be spontaneously inserted into large unilamellar vesicle (LUV), even in the absence of protein machineries or membrane potential. However, detailed interaction...
Visual monitoring of membrane potential would be extremely useful for monitoring cell health and activity in any cultured neuron system. On long time scales, cell health in response to depolarization and/or hyperpolarization would be able to be quantified. If the reporter has sufficient time resolution, action potentials may be resolved, thus allowing for optical recordings from ensembles of neurons...
Dimethylformamide (DMF) is a hydrophilic aprotic solvent often used in peptide coupling, an essential process in the pharmaceutical industry. Its use in biological study is typically in the role of a peptide solvent when evaluating the efficacy of peptides and their effect on the cellular membrane. In evaluating the effect of the peptide gramicidin on neurons of the central nervous system (CNS) of...
Gramicidin is a pore-forming peptide which exhibits lethal properties against a large spectrum of cells. It forms monovalent cation-specific channel in the lipid bilayer of a cellular membrane with limited permeability to anions or polyvalent cations. Both ions and water move through the pore which is formed by the peptide backbone. We detected formation of pores induced by the dimerization of gramicidin...
A reduced order differential model of cardiac Purkinje fibres action potential, with only eight state variables, is presented. Its structure, derived from basic physical principles can be used for the main other cardiac cell types, a useful property for some model-based signal or image processing applications. The electrical activity of cardiac Purkinje fibres is reconstructed using particular values...
A reduced order differential model of cardiac Purkinje fibres action potential, with only eight state variables, is presented. Its structure, derived from basic physical principles can be used for the main other cardiac cell types, a useful property for some model-based signal or image processing applications. The electrical activity of cardiac Purkinje fibres is reconstructed using particular values...
The advent of semiconductor devices with nanoscale dimensions creates the potential to integrate nanoelectronics and optoelectronic devices with a great variety of biological systems. Moreover, the advances in nanotechnology are opening the way to achieving direct electrical contact of nanoelectronic structures with electrically and electrochemically active subcellular structures-including ion channels,...
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