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FISHing for complements: The proteome and the transcriptome in single E. coli cells were determined simultaneously through the use of a YFP fusion library (left, yellow) and an Atto‐594‐labeled complementary probe to the same YFP by fluorescence in situ hybridization (FISH; right, red), respectively. Interestingly, for the 129 highly expressed genes studied, no correlation between transcription and...
DDRT-PCR is an effective and quick method to study differentially expressed genes on the same cells under differently physiological status and different stages. In this study fluorescence DDRT-PCR was used to compare mRNA of peripheral leucocytes of Common Carp stimulated and non-stimulated by mitogens (LPA, ConA and PHA) in different times such as 4 h, 12 h and 24 h. According to bioinformatic technology...
The effects of three structurally related flavonols (kaempferol, quercetin, myricetin) on anti-proliferation and cell cycle arrest in human oesophageal adenocarcinoma OE33 cells were determined and compared by MTT assay and flow cytometry analysis. The results showed that three flavonols not only inhibited proliferation but also induced G2/M arrest in OE33 cells. Moreover, G2/M arrest-inducing activity...
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