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Sequential fractionation of extractive-free maize stems was carried out using two mild alkaline extractions (0.5 and 2M NaOH, 20°C, 24h) before and after endoglucanase treatment. This procedure provided two lignin–carbohydrate fractions (LC1 and LC2) recovered after each alkali treatment. LC1 and LC2 contained 39% and 8% of the total lignin amount, respectively. These two fractions contained structurally...
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