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We described a novel method that combined primer extension and ligation for simultaneously detecting a set of single nucleotide variations. To verify the feasibility of this method, seven synthetic templates containing seven KRAS mutation sites and one template without mutation were detected by four primer extension cycles and one ligation reaction in the present of labeled or unlabeled nucleotides...
Fusion of biological tissues through direct and indirect heating is a growing area of medical research, yet there are still major gaps in understanding this procedure. Several companies have developed devices which fuse blood vessels, but little is known about the tissue's response to the stimuli. The need for accurate measurements of tissue behavior during tissue fusion is essential for the continued...
A novel approach for simultaneous detection of multiple SNPs genotyping by ligation of universal probes on a 3D polyacrylamide gel DNA microarray was reported in this study. In the principle experiment, synthetic single-stranded DNA (ssDNA) templates simulating 3 SNP loci modified with acrylamide group at the 5′-termial were copolymerized with the acrylamide monomer to be immobilized onto one chip...
In this research, a buried suture simulator is developed from attention that there is a most complicated diversity in plastic surgery. Buried suture is one of suture techniques with difficult usage. In comparison with normal suture, buried suture needs more care because it is difficult to handle it. Moreover accuracy of surgical techniques or effect of skill on postoperative deformation are unknown...
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