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The immobilized sucrase capsule of 3.0mm diameter was prepared by chitosan and sodium carboxymethyl cellulose (CMC) ion interaction. The immobilization conditions were optimized as following: The sucrase was dissolved in chitosan solution with the concentration of 2.0%. The solution was dropped into the CMC solution with concentration of 1.5%, and the formed capsules were kept hardening for 24h at...
Intervertebral disc (IVD) degeneration is accompanied by a loss of proteoglycans (PGs) in the nucleus pulposus (NP), resulting in a loss of charged chondroitin sulfate (CS) chains, decreasing mechanical function of the disc. PG mimics may be employed to restore NP functionality. Synthesis of CS bottle brush structures requires immobilization of CS at its terminal end. In this study, we have investigated...
High density gold nanoparticles (GNPs) on indium tin oxide (ITO) film coated glass have been prepared by electrochemical deposition from KAu(CN)2 solution. In this study, suitable applied potential ranges and deposition cycles are chosen to achieve high density GNPs on ITO surface. The potential utility of the high density GNP/ITO substrate are investigated. The superoxide dismutase (SOD) was immobilized...
The nanogap automated permittivity measurement system (APMS) are fabricated as low cost, portable and label free DNA hybridization detection kit. The nanogap capacitor can react as a dna sensor. The difference in dielectric properties of single-stranded DNA (SSDNA) and double-stranded DNA (DSDNA) are allowing the use of permittivity measurement for DNA hybridization detection. In this project, an...
Adsorption based immobilization of amino acids leucine on the surface of undoped polypyrrole (Ppy) is investigated. Calculations are done based on density functional theory using Gaussian03 software and applying GGA exchange-correlation model of PBE (Perdew, Burke, Ernzerhof) with 6-31G(d) basis set. The energy of the Ppy doped with amino acids are minimized with respect to the orientation and distance...
To identify dairy cows suffering from subclinical mastitis, we used the fact that the number of neutrophils increases in raw milk. The neutrophils, a type of white blood cells, produce superoxide (O2). Therefore, the amount of O2 produced in raw milk can be used as an indicator for mastitis. To this end, we fabricated a device consisting of thin-film threee-lectrode systems. A self-assembled monolayer...
This paper presents a novel Ni-Co alloy coated QCM biosensor fabricated by electroplating nickel and cobalt on the surface of 10-MHz AT-cut QCM. The biotin was immobilized on the coated surface by the affinity mechanism of Ni-Co alloy. Then, the immunoreactions can be performed on the QCM biosensors. The results show that the Ni-Co alloy coated QCM biosensor can provide specific binding with His-tagged...
Processed synthetic polymers used in medical textile are used directly or functionalized by immobilization of bioactive molecules. Textile products made by synthetic polymers are durable, resistant to chemical reactions and have inert structures. Textile products made by synthetic polymers usually have not functional groups for immobilization. So modification could be done for formation of functional...
Detection of microbial pathogens in food is the solution to the prevention and recognition of problems related to health and safety. New biomolecular approaches for foodborne pathogen detection are being developed to improve the biosensor characteristics such as sensitivity and selectivity, also which is rapid, reliable, cost-effective, and suitable for in situ analysis. Recently, conducting polymers...
Immobilization of bacteriophage T4 onto the SPEs was achieved by formation of amide bonds between the protein coating of the phage and the electrochemically generated carboxylic groups at the carbon surface. The immobilized T4 were then used to specifically detect target bacteria (E.coli). Impedance measurements (Nyquist plots) show significant shifts in both the real (Zr) and imaginary (Zi) impedances...
A novel optical biosensor technique is being investigated for early detection of myocardial infarction utilizing the distance-dependent chemical transduction method of fluorescence resonance energy transfer (FRET). The FRET process requires two fluorophores termed the donor and the acceptor. When in close proximity, the donor absorbs energy from the excitation source and nonradiatively transfers the...
Summary form only given. In the past 30 years, the author and his colleagues have focused their research on the biomechanics of musculoskeletal soft tissues, ligaments and tendons, in particular. Therefore, in this lecture, the function of knee ligaments, the associated homeostatic responses secondary to immobilization and exercise, and healing of the ligaments will be reviewed. Examples of scientific...
A magnetic bead based immobilization method and pH detection method is applied to the LAPS (light addressable potentiometric sensor) system to detect a pathogenic micro-organism population. Magnetic beads are very small, superparamagnetic particles (0.8/spl sim/5.0 /spl mu/m in diameter) that are able to sustain a magnetic domain under excitation and do not exhibit residual magnetization when the...
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