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Interdigitated electrode based electrical biosensors are prominent in biosensor field. The large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. Listeria monocytogenes is a serious food borne pathogen based bacterium that can cause dangerous disease to human, some infection may result in death. In here, biosensor was prepared from 1μm gap Aluminum...
Immobilization is an important process of biosensor application. The simplest functionalize diamond surface was done by attached biomolecule on diamond surface with chemical linker compound modification. Carboxyl treatment is one choice for diamond functionalization. Methane gas was diluted with hydrogen gas for CH3 termination as an initial functionalization of diamond to transform to carboxyl termination...
This paper presents an overview of the quartz crystal microbalance sensor (QCM) for bacteria application. The QCM sensor can be considered as a representative mechanical transducer for a biosensor device that converts the mass of target analytes into an electrical signal. Microorganisms' detection using QCM sensor can be applied in applications in the field of environment, biomedical area, and food...
Nanomaterials such as silica nanoparticles works as sensor when probe hybridized with the target sequence. Due to their large surface area to volume ratio, they can achieve rapid and low cost reactions. They can conjugate with a variety of biomolecules (Proteins, enzymes, peptides, and DNA) and used as fluorescence biomarkers to monitor biological events or identify and detect biological targets....
For rapid screening nano size biomolecules, a device must be designed in nano sizes, compact and ultra-high sensitive. The most interesting function of the devices is to transduce the binding of biomolecules and devices into electrical signals for further analysis. Over the past few years, nanostructures have emerged as an excellent material to be applied in electrical biosensor. It is because nanostructure-based...
A direct covalent immobilization of glucose oxidase was performed on carboxylated boron doped diamond (BDD) film using 1-Ethyl-3-(dimethylaminopropyl)-carbodiimide (EDC). The carboxylated BDD film deposited using the microwave plasma CVD was obtained by the chemical treatments of oxidization and hydrolysis. The immobilized glucose oxidase was confirmed by a spectrophotometric assay to have the enzyme...
A direct covalent immobilization of glucose oxidase was performed on carboxylated boron doped diamond (BDD) film using 1-Ethyl-3-(dimethylaminopropyl)-carbodiimide (EDC). The carboxylated BDD film deposited using the microwave plasma CVD was obtained by the chemical treatments of oxidization and hydrolysis. The immobilized glucose oxidase was confirmed by a spectrophotometric assay to have the enzyme...
Adsorption based immobilization of amino acids leucine on the surface of undoped polypyrrole (Ppy) is investigated. Calculations are done based on density functional theory using Gaussian03 software and applying GGA exchange-correlation model of PBE (Perdew, Burke, Ernzerhof) with 6-31G(d) basis set. The energy of the Ppy doped with amino acids are minimized with respect to the orientation and distance...
This paper concerns the development of a potentiometric transduction circuit for a piezoresistive MEMS sensor to detect human stress. The biosensor is comprised of a bioreceptor and a transducer. The sensing principle is based on immobilization of the bioreceptor to produce a biochemical reaction. The novel piezoresistive microcantilever sensor integrated with a transduction circuit converts this...
A novel optical biosensor technique is being investigated for early detection of myocardial infarction utilizing the distance-dependent chemical transduction method of fluorescence resonance energy transfer (FRET). The FRET process requires two fluorophores termed the donor and the acceptor. When in close proximity, the donor absorbs energy from the excitation source and nonradiatively transfers the...
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