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Alkali pretreatment of sugarcane tops was carried out with 3% NaOH for 60 min at 121 °C in a laboratory autoclave. The effect of solid loading, enzyme loading, incubation time and surfactant concentration on enzymatic saccharification was studied using a response surface method according to Box–Behnken design. Under optimized conditions 77.5% sugar was recovered from the pretreated biomass. This yield...
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