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The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. As a prokaryotic adaptive immunity system, that was originally adapted from the bacterial Type II CRISPR, CRISPR-Cas9 uses a non-coding RNAs. Those RNAs guides Cas9 nuclease, which in turn induce site-specific DNA cleavage at a specific locations in genome. Such mechanism gives an opportunity...
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