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An engineered Streptomyces lydicus strain with 6-phosphofructokinase gene (pfkA1) over-expression was constructed by recombinant DNA technology. Biomass, pH value, glucose and streptolydigin concentrations were measured and the fermentation kinetics were calculated. The cell growth and glucose consumption of the engineered strain was faster in the early period, and then slow down in the later stage...
In a fermentation system, the biomass balance and the substrate mass balance are coupled by a nonlinear growth rate condition. In order to control this nonlinear process, many researchers assume that both substrate and biomass concentration are measurable. In practice, only online measurement of substrate concentration is readily available. Estimating the biomass concentration can be done but it is...
Utilizing the ability simple in application and quick in convergence of quantum delta-potential-well-based particle swarm optimization (QDPSO) algorithm and the high generalization ability of support vector machine (SVM), selecting the appropriate state variables, a dynamic time-varying model has been built. Using the model and algorithm to per-estimate some biochemical state variables which can not...
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