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To produce various neural cell types from human pluripotent stem cells (hPSCs), the derivation of neural stem cells (NSCs) would be the first step. We have developed an efficient neural induction medium that can convert hPSCs into NSCs in 1 week and also skip the tedious time consuming embryoid body formation. Neural induction medium works for hPSCs cultured either in feeder contained or feeder‐free...
Neural stem (NS) cells can provide a source of material with potential applications for neural drug testing, developmental studies, or novel treatments for neurodegenerative diseases. Herein, the ex vivo expansion of a model system of mouse embryonic stem (mES) cell‐derived NS cells was characterized and optimized, cells being cultivated under adherent conditions. Culture was first optimized in terms...
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