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Different instances of a biological macromolecular complex need not share the same 3-D electron scattering distribution function due to stoichiometric variability, flexibility and vibrations, etc. Cryo electron microscopy provides 2-D images of each of many such complexes where each image is roughly a projection. A statistical estimation problem is described and demonstrated for determining a statistical...
Two algorithms are described for electron microscopy tasks: (1) 3-D reconstruction of an infectious bacteriophage, including the tail, based on a set of single-particle cryo electron microscopy images of identical phage particles with one image per particle and (2) restoration, resolution enhancement, and classification of spherical viruses in a cell based on a tomographic reconstruction of the entire...
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