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Mammalian adipose tissue derived stem cells have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money...
The crosslinks and microstructure of cartilage are poorly understood and there are many different techniques to isolate chondrocytes from their extracellular matrix. The purpose of this study is to obtain an optimised protocol to harvest the highest number of viable cells from a particular species of cow, Bos indicus, which has not been previously reported. Therefore, this would allow the preparation...
Microporous polycaprolactone (PCL) matrices containing lysozyme, collagenase and catalase respectively with molecular weight covering a wide range from 14.3 to 240kDa were produced by a novel method involving rapid cooling of particle suspensions in dry ice. The enzyme loading efficiency (lysozyme (50%), collagenase (75%) and catalase (90%)) depended on the enzyme molecular weight and the non-solvent...
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