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Biofuels and biomaterials, produced from lignocellulosic feedstock, require facile access to cellulose and hemicellulose to be competitive with petroleum processing and sugar-based fermentation. Physical-chemical barriers resulting from lignin complicates the hydrolysis biomass into fermentable sugars. Thus, the amount of lignin within a substrate is critical in determining biomass processing. The...
In the Arabidopsis mutant irx3, truncation of the AtCesA7 gene encoding a xylem-specific cellulose synthase results in reduced cellulose synthesis in the affected xylem cells and collapse of mature xylem vessels. Here we describe spectroscopic experiments to determine whether any cellulose, normal or abnormal, remained in the walls of these cells and whether there were consequent effects on other...
Barley straw was separated mechanically into leaf and stem fractions. Their 13 C NMR spectra were recorded at low field (25 MHz) using magic-angle spinning and cross-polarisation (CP-MAS) under conditions optimised for quantitation of lignin. The stem fraction contained more lignin with a higher proportion of ether-linked syringyl residues. The leaf fraction contained more phenolic acids and...
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