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The imaging rate of conventional atomic force microscopy (AFM) is too low to capture the dynamic behavior of biomolecules. To overcome this problem, we have been developing various devices and techniques, including small cantilevers and high-speed scanners. The feedback bandwidth in the tapping-mode now exceeds 100 kHz and hence the maximum possible imaging rate reaches 25 frames per sec (fps). Importantly...
We report a novel electrical DNA biosensor, which consists of gold (Au) nano islands and SWCNT net on top of a semi-concentric Au electrode array (also referred to as the CGi). The real time measurement in TE buffer solution in a wide range of the target ss-DNA concentration down to fM (10-15 M) was performed to check the detection limit. The CGi, functionalized with 25mer probe ss-DNA (5'-HSC6-C18...
In this paper we have proposed and analyzed a novel integrated optic racetrack resonator based biosensor. The device is capable of distinguishing compressive and tensile stresses on a cantilever due to conformational changes of protein. We have considered Bovine Serum Albumin (BSA) and Immunoglobulin G (IgG). The change of surface stress upon adsorption of IgG is compressive and of BSA is tensile...
The fluorescent tagging of biomolecules can have substantial effects on the charge distribution and translocation of the molecules through nanochannels. We show that the diffusion and calculated flux of translocating protein molecules through nanochannels are strong functions of the tags used. The size of the nanopore channels of a membrane also affect whether the channels provide facilitated transport...
A sensitive immunosensor based on charge detection is developed for proteins. Its aim is to select and quantify low protein concentrations in a complex environment. This highly sensitive sensor is a SGFET (Suspended Gate Field Effect Transistor) whose gate is suspended at 500 nm high above the silicon channel and embedded in silicon nitride. This electrical insulation and the general design of the...
Micro-strip-line-based THz sensors were applied to the characterization of liquid water containing a protein (albumin) and amino-acid (arginine). Density-dependent attenuation of the THz waves was clearly observed for both the cases.
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