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The current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the...
In this work, cell electrophoresis, measuring the electrophoretic mobility of cells, was used to investigate the variation of surface charge property of cells after cultured on different polymer membranes. HepG2 cell line and neural stem/progenitor cell were used as the model cells. The polymer biomaterials used in this study included polyvinyl alcohol (PVA) and polyvinylidene fluoride (PVDF). Taken...
Current cell detection techniques are antibody staining of specific protein markers, morphometric parameters and transgenic markers. These assays are often qualitative and do not quantitatively define the outcome of a cell progression during differentiation. Consequently, we propose to characterize the mechanical behavior of embryonic stem cell, which will predict its stage of differentiation during...
The human mesenchymal stem cells can differentiate into cardiomyocytes, osteoblasts, chondroblasts, adipocytes, endothelial, hepatocyte and neuron cells, when hMSCs are exposed to certain physical and chemical stimulants. It is hoped that the differentiation of hMSCs into certain cell types in certain in-vitro conditions can provide for treatment of the certain disease and pathologic situation. The...
The stromal compartment of the normal breast microenvironment is abundant in adipose progenitor cells. These cells experience differential mechanical cues during mammary tumorigenesis; however, it is largely unknown whether tumor-mediated reduction in extracellular matrix (ECM) compliance alters the behavior of adipose progenitor cells. To investigate this possible relationship we have developed an...
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