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To investigate whether vascular endothelial growth factor (VEGF) increases cancer cell adhesion to intact microvessels, we measured adhesion rates of MDA-MB-435s human breast cancer cells in the postcapillary venule of rat mesentery by in vivo video microscopy. An individual postcapillary venule in the mesentery was cannulated via a glass micropipette filled with cancer cells and 1% BSA Ringer solution...
Targeted ultrasound contrast agents (USCA) can be used as molecular imaging agents to visualize the expression of endothelial molecules (biomarkers). In a murine tumor model, three biomarkers for tumor vasculature were imaged using targeted USCA and the high frequency ultrasound system Vevo 770. Three biomarkers were targeted: VCAM-1, selectins (P and E), and VEGF receptors. Targeting was determined...
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