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Mixed neuronal and glial cell spinal cord cultures from neonates express ADP sensitive P2Y 1,12&13 receptors. ADP (10μM) evoked increases in intracellular calcium that were essentially abolished by the P2Y 1 receptor antagonist MRS2179 (10μM), responses were also absent in preparations from P2Y 1 receptor deficient mice however UTP (100μM) evoked calcium rises were unaffected...
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